Work Package 2
In vitro investigations of brain endothelial cell receptor internalization and trafficking pathways
Principal Investigator: Morten Schallburg Nielsen, Department of Biomedicine, Aarhus University
This work package deals with analysis of the receptor-mediated transport systems in brain endothelial cells, which have the potential to mediate transcytosis of nanocarriers and antibodies. Previous studies using the Transferrin receptor and LRP1 (Low density lipoprotein receptor-related protein1) have succeeded in transporting drugs across the endothelium.
In this work we will characterize different subcellular trafficking routes of known and novel receptors in brain endothelial cells. Currently, we are focusing on the recycling receptor Transferrin receptor and the retrograde transported mannose-6-phosphate receptor. The work will furthermore include a detailed classification of the endo-lysosomal system and functional internalization mechanisms in in vitro models of the blood-brain barrier.
The aim is to understand the receptor-mediated transcytosis mechanism in details in order to select the optimal receptor system for drug delivery to the brain.
Our studies are primarily based on state-of-the-art imaging systems such as confocal, spinning disk, high content screening and TIRF microscopy in combination with in vitro blood-brain barrier models.
|Andrea Tóth||Assistant firstname.lastname@example.org|
|Morten Schallburg Nielsen||Associated email@example.com|
|Mikkel Roland Holst||PostDocfirstname.lastname@example.org|
|Sarah Christine Christensen||PhD email@example.com|
|Simone Elstrøm Nielsen
PhD defence: Sarah Christine Christensen
May 06, 2020
Sarah Christine Christensen will defend her PhD thesis...
PhD defence: Nikolay Kutuzov
Jan 07, 2020
Nikolay Kutuzov will defend his PhD thesis...
Søren Grubb wins the “Best Presentation Award”
Nov 28, 2019
Søren Grubb, Assistant Professor from Martin Lauritzen Lab, received the “Best Presentation Award”...
RIBBDD BIANNUAL MEETING, NOVEMBER 2019
Nov 21, 2019
November 19-20, 45 members from the RIBBDD network met at The Mærsk Tower in Copengahen...
PhD defence: Charlotte Goldeman Andersen
Jul 02, 2019
Charlotte Goldeman Andersen will defend her PhD theisis...
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