Work Package 5

Januar 2014-160
Januar 2014-151
Januar 2014-153
Januar 2014-144

In vitro models of the neurovascular unit

Principal Investigator: Birger Brodin, Drug Transporters in ADME, Department of Pharmacy, University of Copenhagen

  • Investigate receptor expression and receptor-mediated transport in bovine in vitro models of the blood-brain barrier.
  • Investigation of blood-brain barrier permeability properties and localization of tight-junctions and receptors during oxygen-glucose deprivation (OGD)/reperfusion treatment, in both contact-coculture and monoculture in vitro
  • Investigate intercellular communication in the neurovascular unit and in particular elucidate the role of ATP in activation of specific purinergic receptor types and changes in intracellular calcium levels in primary bovine pericytes.
  • Generate and characterize an in vitro model of the blood-brain barrier using human induced pluripotent stem cells in co-culture with other cell types of the neurovascular unit, such as astrocytes and pericytes. Such a model would have a wide array of research opportunities and would be an important tool in testing e.g. active drug transport and cell-to-cell interactions at the blood-brain barrier.

Granted participants:

        Name Title E-mail
      Birger Brodin       Professor
      Hans Christian Cederberg Helms       Assistant Professor
      Charlotte Goldeman Andersen       PhD student
      Nana Isabella Svane       Scholar student        

Project contributors:

        Name Title E-mail
      Mie Kristensen       Assistant Professor
      Lasse Saaby       Research Assistant
      Burak Ozgür       PhD student
        Patrick Frøslev       Master's student
        Emma Lisa Al Humaidan
      Master's student        
        Mette Louise Sommer
      Master's student        
        Frederikke Jørgensen
      Master's student        
        Thomas Bucholz       Master's student        
        Ahmed Al-Robai       Master's student        
        Sven Barbara Hinca       Master's student