Work Package 4
Signaling at the neurovascular unit and blood-brain barrier control
Principal Investigator: Professor Martin Lauritzen, Neuronal Signaling Laboratory, Department of Neuroscience and Pharmacology, University of Copenhagen
The work package is divided into five parts all focused on elucidating function and pathology of the neurovascular unit and the blood-brain barrier:
- We will develop a method for quantitative determination of BBB permeability using living mice and two-photon microscopy. Currently, most assessment of BBB permeability in animals in vivo is based on isotope methods with one measurement in time in each animal. The goal is to enable us to determine BBB permeability changes in real time at the single capillary level continuously in experimental animals.
- We will examine pericyte-astrocyte and astrocyte-pericyte communication with focus on calcium signals in both cell types using genetically engineered mice expressing genetically encoded calcium indicators.
- We will develop a stroke model in mice based on middle cerebral artery occlusion. The methods developed in Project 1 will be used to quantify BBB breakdown at the level of single capillaries in the necrotic area, in the penumbra and in the adjacent normal tissue over time.
- We will examine whether BBB breakdown in focal ischemia can be ameliorated by blockade of astrocyte and pericyte purinergic receptors, and by inhibitors of MMP9 based on nanoparticle mediated delivery
- We will examine diffusion properties of nanoparticles in the brain extracellular space, and the influence of convection and diffusion in the paravascular spaces on the distribution limitations for nanoparticles and macromolecules in brain.
|Krzysztof Kucharz||Assistant firstname.lastname@example.org|
|Mette Mathiesen Janiurek||PhD email@example.com|
|Oleg Zhukov||PhD firstname.lastname@example.org|
PhD defence: Sarah Christine Christensen
May 06, 2020
Sarah Christine Christensen will defend her PhD thesis...
PhD defence: Nikolay Kutuzov
Jan 07, 2020
Nikolay Kutuzov will defend his PhD thesis...
Søren Grubb wins the “Best Presentation Award”
Nov 28, 2019
Søren Grubb, Assistant Professor from Martin Lauritzen Lab, received the “Best Presentation Award”...
RIBBDD BIANNUAL MEETING, NOVEMBER 2019
Nov 21, 2019
November 19-20, 45 members from the RIBBDD network met at The Mærsk Tower in Copengahen...
PhD defence: Charlotte Goldeman Andersen
Jul 02, 2019
Charlotte Goldeman Andersen will defend her PhD theisis...
There are no upcoming events at this time.